Coding
Part:BBa_K1959000:Design
Designed by: Junyu Chen Group: iGEM16_SCAU-China (2016-10-03)
Modification of Phytoene synthase (PSY)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1045
Illegal NheI site found at 1164 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 105
Illegal AgeI site found at 956 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence has been optimized for expression in Orazy sativa. Nucleotide substitution: g117c, gc195cg, g372a, c529a, t753a, a769t, a798g, g819a, t927c, a1031c. The numbers stand for the location where nucleotide subititution carried out. Nucleotide fellow the numbers is the mutant result.
Source
The complete CDS sequence can be obtained from Genebank. Genebank accession:U32636